2002 CTOS
Annual Meeting Oral Presentations — Biology
DIFFERENTIAL
GENE EXPRESSION IN LEIOMYOSARCOMA
[Abstract
ID: 24]
Category:
Biology
Presentation:
Oral
Authors:
Keith M Skubitz1, Amy P. Skubitz1
Author Institutions:
1University of Minnesota, MN, United States
Presenter:
Keith M Skubitz
skubi001@tc.umn.edu
Correspondent:
Keith M Skubitz
skubi001@tc.umn.edu
Minneapolis MN United States 55455
Ph: 612-625-5411
Fax: 612-626-1441
Objectives: Soft
tissue sarcomas are a heterogeneous group of malignancies of mesenchymal
origin, the causes of which are unclear. In this study gene expression
in leiomyosarcomas, myometrium, and uterine leiomyomas was examined.
Methods: RNA was prepared and gene expression was determined
at Gene Logic Inc. (Gaithersburg, MD) using Affymetrix GeneChip®
U_95 arrays containing approximately 12,000 known genes and 48,000
ESTs. Gene expression analysis was performed with Gene Logic Gene
Express® software. Differences in gene expression were quantified
as the fold change in gene expression in leiomyosarcomas compared
with normal myometrium, and uterine leiomyomas.
Results: A number of genes were differentially expressed
in these sample sets, and these genes were analyzed for their expression
in a variety of other normal and diseased tissues. Some of the genes
identified were over-expressed only in leiomyosarcomas among the
tissues examined, and some were over-expressed in other tissues
as well
Conclusions: We conclude that differences in gene expression
can be detected between leiomyosarcomas and uterine leiomyomas,
and normal myometrium, and that these changes in gene expression
may yield clues to the pathophysiology of this malignancy.
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