2002 CTOS Annual Meeting Oral Presentations — Pathology

EXPRESSION OF THE IHH/PTHRP AND BMP RECEPTORS HAS DIAGNOSTIC RELEVANCE IN CARTILAGINEOUS LESIONS
[Abstract ID: 17]

Category: Pathology

Presentation: Oral

Authors: Luca Sangiorgi¹, Joerg Warzecha¹, Enrico Lucarelli¹, Licciana Zanella¹, Marco Alberghini¹, Veronica Maini¹, Elena Pedrini¹, Patrizia Bacchini¹, Franco Bertoni¹, Piero Picci¹

Author Institutions: ¹Rizzoli Orthopedic Institute, Italy

Presenter: Luca Sangiorgi
luca.sangiorgi@ior.it

Correspondent: Luca Sangiorgi
luca.sangiorgi@ior.it
Bologna Italy 40136
Ph: +39-051-6366519
Fax: +39-051-6366761

Objectives: Indian Hedgehog (and its downstream target) and Bone Morphogenic Proteins have shown to play a relevant and independent role in condrocytes proliferations and regulations during embryonic development. Aim of this study is to evaluate if the expression of these molecules can be used for diagnostic classification of cartilaginous lesions.

Methods: cartilagineous lesions were analysed by immunohistochemistry with the antibodies for Indian Hedgehog (Ihh), Parathyroid Hormone related peptide (PTHrp), Bone Morphogenic Protein Receptors type 1 A (BMPR-IA), type 1B (BMPR-IB) and type 2 (BMPR-II) receptors. Staining was evaluated by 4 investigators independently.

Results: We observed a distinct pattern of expression of these proteins in specific subset of cartilagineous lesions. Ihh and BMPR-II expression were found in 4,9% and 5,1% of osteochondromas. In contrast, the same antigens were expressed in 54,1% and 48,3% of peripheral chondrosarcomas. In central condroid lesions, two different proteins, PTHrp and BMPR-IB expression showed different pattern in enchondromas (11,1% and 25%) and grade I chondrosarcomas (67,7% and 79,5%).

Conclusions: Immunohistochemical detection of these proteins could identify different subset of cartilagineous lesions and could be useful for diagnostic purposes.